The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. Cells can be assayed 12 to 24 hours post-transfection instead of the typical 48 to 72 hours. This method is particularly suited for the generation of transient transfectants due to the short incubation time required.
However, stable transfectants have also been generated with increased efficiency.
However, stable transfectants have also been generated with increased efficiency.
Jul 02, 2015 The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. 2016 ford fusion owners manual. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. Cells can be assayed 12 to 24 hours post-transfection. Transfection Transfection is the process by which nucleic acids are introduced into mammalian cells. Protocols and techniques vary widely and include lipid transfection and chemical and physical methods such as electroporation. Expertise in cellular and molecular biology is behind the Invitrogen™ product portfolio of DNA reagents and consumables, which are the most cited and trusted. ViraPack Transfection Kit 3 TRANSFECTION PROTOCOL Note Please read the entire transfection protocol before beginning. See the final page of this instruction manual for a condensed, quick-reference protocol that can be detached and used when detailed instructions are no longer required. Calcium phosphate transfection is one of the most commonly used methods to deliver plasmid DNA into mammalian cells. Due to its highly consistent pH and salt concentration, our CalPhos calcium phosphate transfection kit gives you the consistent transfection efficiency that you cannot always achieve with home brew versions.
For Research Use Only. Not for use in diagnostic procedures.
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Features
- Increases transfection efficiencies by up to 100-fold
- Increases yield of stable transformants
- Perform multiple micro-transfections in 96-well microtiter plates with as little as 5,000 cells per well
- Modified bovine serum increases number of cells transfected
- Efficient, rapid and simplified DNA transfection of mammalian cells
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent mammalian transfection kits produce up to 100-fold higher yield of stably infected colonies than standard CaPO4. The kits are optimized for 30 transfections in 100 mm tissue culture dishes using a CaPO4 protocol to produce stable transfectants, and for 30 transfections using a DEAE-dextran protocol for transient transfections.
Agilent mammalian transfection kits produce up to 100-fold higher yield of stably infected colonies than standard CaPO4. The kits are optimized for 30 transfections in 100 mm tissue culture dishes using a CaPO4 protocol to produce stable transfectants, and for 30 transfections using a DEAE-dextran protocol for transient transfections.
Calphos Mammalian Transfection Kit
For Research Use Only. Not for use in diagnostic procedures.
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